We see that electrospun wound matrices are unique drug delivery systems that need special analytical techniques in addition to the established methods for the evaluation of their performance. Thus, in parallel with the development of wound matrices we also develop methods and biorelevant models which enable to understand their behavior in biological environment, safety and efficacy, unraveling the structure and activity relationships, and more importantly help us to understand the wound matrix-bacteria-eukaryotic cell interactions in wound environment. Better understanding of these relationships helps to guide rational design of safe and effective electrospun fibrous wound dressings.
For example, conventional drug release testing methods do not reflect the wound environment adequately and cause difficulties for correlating and predicting biological activity from the dissolution data. We have developed biorelevant hydrogel-based drug release and diffusion assays using extraction/HPLC, UV-imaging and bacterial bioreporters for detection. The latter provides an additional benefit of simultaneously monitoring antibacterial activity of the released drug. Also, we put special emphasis on designing wound infection models with sessile bacteria in biofilm as it is recognized as a major culprit in therapeutic failure of non-healing wounds.